(2) Preparation of antiserum â‘ The amount of antigen required is generally 1-10mg / mL. â‘¡The injection methods of immune route antigen include subcutaneous injection, intramuscular injection, intraperitoneal injection, intravenous injection and lymph node injection. Among them, intravenous injection is the most simple and convenient. No adjuvant emulsification is needed, and the peak of antibody production occurs earlier, generally about one week, and the blood can be tested one week after injection. During the immunization process, attention should be paid to: a. The adjuvant can only be used now: b. The blood collection time should not be dragged too long. Because the longer the immunization time, the more antibodies that cross-react. â‘¢ The separation of antiserum can be performed when the satisfactory titer is obtained. Place the collected blood in a slanted surface at 37 ° C in an incubator for 2h. Then put it in the refrigerator at 4 ℃ overnight. The next day, pipette the antiserum to remove the precipitate by centrifugation (2000-3000r / min, 20min). Then determine the titer and identify the purity. The purity is measured by ultraviolet spectrophotometer or Folin's colorimetric method. The antiserum is stored at a low temperature of -25 ° C. â‘£ There are generally two methods for determining the titer of antiserum, namely the test tube precipitation method and the immunodiffusion method. For the antiserum used by immunoenzyme technology, it must have a certain potency, the test tube precipitation method should not be less than 1: 2, the immunodiffusion method is not less than 1: 2, and a monovalent antiserum is required. (3) There are three main methods for the collection and preparation of predators: â‘ Squeeze the stomach contents on good quality filter paper, but the protein is easy to combine with cellulose, if it is stored for a long time, it is difficult to elute: â‘¡ will be checked The predation is enclosed in gelatin capsules, and a desiccant is added to separate the insect body from the capsule: â‘¢ Store the collected predators at a low temperature (such as -40 ° C) as quickly as possible. The first two methods are suitable for mailing and long-distance transportation, the latter method can be stored for a long time. All methods need to be kept dry. For larger predators, the digestive tract can be separated, and small animals such as ants, snails, and spiders can be whole insects. (4) The enzyme-linked antibody is prepared according to the simplified method of glutaraldehyde. Material: Ceramic orchid pots are typically crafted from clay-based materials that are fired at high temperatures to achieve durability and strength. They can vary in texture, from smooth and glossy to rough and matte, depending on the specific ceramic composition and finish. Design: These pots often feature a wider base and narrower opening, providing stability for the orchid plant while allowing for proper aeration and drainage. The design may include decorative elements such as embossed patterns, painted motifs, or textured surfaces, enhancing the aesthetic appeal of the pot. Size: Ceramic orchid pots come in a range of sizes to accommodate different orchid species and growth stages. They can vary from small pots suitable for single orchid plants to larger containers capable of holding multiple plants or larger root systems. Color: The color of ceramic orchid pots can vary widely, offering options to complement various orchid flower colors and interior or outdoor décor themes. Common colors include white, beige, terracotta, green, blue, and various shades of earth tones. Drainage Holes: Proper drainage is crucial for orchid health, and ceramic orchid pots typically feature multiple drainage holes at the bottom to prevent waterlogging and root rot. These holes allow excess water to escape freely while providing adequate aeration to the orchid roots. Saucer or Tray: Some ceramic orchid pots come with matching saucers or trays to catch excess water drainage, protecting surfaces from moisture damage and providing a tidy appearance. Insulation: Ceramic pots offer some insulation against temperature fluctuations, which can be beneficial for orchids, helping to maintain stable root temperatures in varying environmental conditions. Longevity: Ceramic orchid pots are known for their durability and can last for many years with proper care. They are resistant to fading, cracking, and weathering, making them a reliable choice for long-term orchid cultivation. Overall, ceramic orchid pots blend functionality with aesthetics, providing a stylish and practical solution for growing orchids indoors or outdoors while enhancing the beauty of the plants they contain. Orchid Pot, Ceramic Orchid Pot, Glazed Orchid pot Yixing Bocai Pottery Co.,Ltd , https://www.bocaipottery.com