Negative staining method-Haibo Biology

(1) Production: Take a piece of clean glass slide, add a drop of distilled water, take a small amount of the bacteria of Bacillus colloidium and put it in the water drop to mix and spread.

(2) Drying: Put the smears in the air to dry or dry them with a cold air blower. ? (3) Dyeing: Add red staining solution on the painted surface for 2 ~ 3min. (4) Washing with water: Wash the red dye solution with water. ? (5) Dry :. Dry the stained sheet in the air or dry it with a cold hair dryer. (6) Melanin: Add a small drop of melanin on the left side of the stained surface, gently touch the melanin with a smooth-edged slide to spread the melanin along the edge of the slide, and then drag to the right, Make melanin a thin layer on the painted surface and quickly air dry.

(7) The microscopic examination first observes with low magnification lens and then with high magnification lens.

Results: The back view was gray, the bacteria was red, and the capsule was colorless and transparent.

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