1. Preparation record of culture medium Each time the medium is prepared, there should be a record, including the name of the medium, the formula and its source, and the brand of various ingredients, the final pH value, the temperature and time of sterilization, the date of preparation, and the preparer. A copy of the record should be copied, the original record should be kept for future reference, and the copy record should be stored with the prepared medium to prevent confusion. 2. Weighing of medium components The various components of the culture medium must be accurately weighed and care must be taken to prevent confusion. It is best to complete them at one time without interruption. The formula can be placed on the side. After each ingredient is weighed, a mark is made on the formula, and the medicines to be weighed are collected at once, and placed on the left side. After each weigh is completed, it is moved to Right. After the weighing is complete, an inspection should be carried out. 3. Mixing and melting of the components of the culture medium The indicator should be added after adjusting the pH value; after boiling, add enough water;? The medium cannot be scorched, and the coked cannot be used. 4. Calibration of medium pH After sterilization, the pH value will decrease by 0.1 to 0.2. When the pH value of the medium is corrected, it should be 0.1 to 0.2 higher than the actual value; after the pH adjustment, the medium should also be boiled. 5. Filtration and clarification of culture medium Liquid media can be filtered with filter paper Agar medium can be filtered with a double layer of gauze with a thin layer of absorbent cotton in between Inclined surface: 1/5 tube semi-solid medium: 1/3 tube? High-level inclined surface: 1/4 ～ 1/3 tube plate: 13 ~ 15ml 7. Sterilization of culture medium (1) Medium containing sugar or gelatin: sterilize at 113 ° C for 15 minutes or 115 ° C for 10 minutes. (2) Sugar-free medium: Sterilize at 121 ℃ for 15-20 minutes. (3) Blood, body fluids, antibiotics, etc. are drawn by aseptic technique and then added to the medium cooled to about 50 ° C. (4) The agar slant medium should be taken out after cooling to 55 ° C-60 ° C after sterilization, and then placed into an appropriate slope. 8. Quality test of culture medium (1) If it is found to be broken, water infiltrated, abnormal color, cotton plug is contaminated with medium, etc., it should be picked out and discarded. And determine its final pH. (2) Put all the culture medium in a 36 ± 1 ° C incubator for overnight cultivation, if any bacteria growth is found, discard it. (3) Inoculate 1 or 2 tubes or flasks of culture medium with relevant standard strains and cultivate for 24 to 48 hours, such as sterile growth or poor growth. The reason should be traced and repeated inoculation. If the result is the same as before, the batch of medium should be discarded and cannot be used. 9. Storage of culture medium (1) The basic medium cannot exceed two weeks (2) The biochemical test medium should not exceed one week, (3) Selective or differential culture is best used on the same day, and the poured plate medium should not exceed 3 days. ABLE Fire Pit Logs, made of light ceramic raw materials or cement materials, light weight but strong not easy to break, hand-painted, natural colors and amazing details & realistic shapes, easy turn redness in the fire, can enhance the fireplace flame, all made of inorganic materials, odorless, fire-resistant temperature >900℃, product can be used for a long time, with reasonable price. Fire Pit Logs,Ceramic Logs For Fire Pit,Fire Pit Wood Logs,Smokeless Logs For Fire Pit Yixing Able Ceramic Fibre Products Co., Ltd , https://www.ablegaslogs.com
ABLE also have advanced molding technology for ceramic log called cast log, it is lighter, stronger, fuller details, and glowing area is larger in the fire.ABLE can supply many kind of log accessories for fireplace, such as pebbles, coals, charcoals, pine cones, maple leaves, aglow ember, top-grade rock wool, stained vermiculite, small coal chips, barks, balls and hollow ball, ceramic pitted pebble and rough Lava rock that won't burst in outdoor fire pit, Pumpkin lamp, Skull and bone for Halloween Decor fire, fireplace log grate etc.
ABLE factory is located in Yixing City, Jiangsu. It is a well-known production base for fire resistant insulation materials and environmental protection products. Yixing is also a city has developed commerce and convenient transportation. Our company sincerely hopes to cooperate with professional customers, and welcome to visit our factory!