Experimental principle: This kit uses the double antibody sandwich method to determine the level of human amyloid 1-42 (Aβ1-42) in the specimen. Microporous plates were coated with purified human β-amyloid 1-42 antibody to prepare solid-phase antibodies, and β-amyloid 1-42 was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled Aβ1-42 The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with β-amyloid 1-42 (Aβ1-42) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human amyloid 1-42 (Aβ1-42) in the sample was calculated by a standard curve. Particle Board,Particle Board Chipboard,furniture Particle Board,Home Decoration Particle Board Shandong Yami Import and Export Co., Ltd. , https://www.yamiwood.com