Anaerobic bacteria cannot grow under aerobic conditions. To cultivate anaerobic bacteria, an anaerobic environment must be created. Usually, a reducing agent is added to the culture medium, or free oxygen in the environment is removed by physical or chemical methods to reduce the redox potential. Such as blister medium, sodium thioacetate medium, bovine heart and brain infusion medium, etc. There are many commonly used anaerobic culture methods, which can be selected according to the actual situation. Writing Desk,modern writing desk,home office desk,minimalist writing desk Foshan Poesy Furniture Co., LTD. , https://www.poesy-furniture.com
1. The anaerobic tank method is used to inoculate the specimen plate or liquid culture medium test tube, which can be put into the anaerobic tank for cultivation. The anaerobic tank is a common drying tank. The bacteria grow out.
2. The anaerobic bag (Bio-bag) is to create an anaerobic environment in the plastic bag to cultivate anaerobic bacteria. The plastic bag is transparent and airtight, and contains a gas generating tube (sodium bicarbonate solid with sodium borohydride and 5% citric acid ampoule), Meilan indicator tube, palladium catalyst tube, and desiccant. After putting the inoculated plate, try to squeeze out the air in the bag, and then seal the bag mouth. Break the gas generating tube first, then break the Meilan indicator tube, and create a gas-free environment in the named bag within half an hour. If no mutation indicates that the bag has reached an anaerobic state, it can be incubated.
3. Anaerobie glove box (Anaerobie glove box) is by far the internationally recognized one of the best instruments for cultivating anaerobic bacteria. It is a closed large metal box with a transparent panel made of plexiglass in front of the box. There are two gloves on the board, which can be operated inside the box through the gloves. There is an exchange room on the side of the box, which has two doors inside and outside. The inner door is closed first. To put things into the box, first open the outer door, put it into the exchange room, close the outer door for pumping and ventilation (H2, CO2, N2) to achieve anaerobic status, then reach into the glove to open the exchange room door and open the item Move into the box and close the inner door. Maintaining the anaerobic state in the tank is also the principle of using the hydrogen in the aeration to catalyze the synthesis of water under the catalysis of palladium and the residual money in the tank. The box can adjust the temperature. It is attached to the incubator or the incubator itself. It can also be placed in a dissecting microscope for easy observation of anaerobic bacterial colonies. This anaerobic box is suitable for anaerobic bacteria mass cultivation research. The base can be put into pre-reduction and anaerobic sterility test. The suction, inflation, anaerobic environment and temperature of the metal hard-wall anaerobic box are automatically adjusted.
4. Anaerobic box: The principle is the same as the anaerobic bag, and there are finished products for sale.
5. Biological oxygen consumption method: put organisms (mostly plants) in a closed container, which consumes oxygen and produces carbon dioxide at the same time for the growth of bacteria. I have not seen.
6. Pyrogalacid method: Spread gauze or filter paper on a clean glass slide, evenly sprinkle pyrogallic acid, and then mix it with NaHCO3 powder or NaOH solution to quickly reverse the plate inoculated with bacteria On the top, the edges are sealed with melted white wax, creating an enclosed space. The pyrotechnic acid consumes oxygen after reacting with alkali. This method is simple for the cultivation of anaerobic bacteria that are not strictly anaerobic. If there is Clostridium.
7. Blister medium: It is an anaerobic culture method without special equipment. The blisters and broth are loaded into large test tubes, and the liquid surface is sealed with petroleum jelly, resulting in an anaerobic environment.