Recently, researchers from the Chinese University of Hong Kong and the Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences have successfully used genetic engineering transcription activator-like effector nucleases (TALENs) technology to obtain highly efficient targeted gene destruction in Xenopus embryos. The related paper "Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs)" was published in the Proceedings of the American Academy of Sciences (PNAS) on October 8. Researchers Yonglong Chen from the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, and Hui Zhao, Christopher HK Cheng from the Chinese University of Hong Kong, and Igor B. Dawid from the National Institutes of Health are co-corresponding authors . TALENs is an enzyme that can target specific DNA sequences. It recognizes specific DNA base pairs with the help of TAL effector, a natural protein secreted by plant bacteria. TAL effectors can be designed to recognize and bind all DNA sequences of interest. TALENs are generated by adding a nuclease to the TAL effector. TAL effect nucleases can bind to DNA and cut DNA strands at specific sites to introduce new genetic material. Compared with traditional zinc finger nuclease (ZFNs) technology, TALENs have a unique advantage: TALENs are cheaper and more effective. Longer gene sequences can be targeted and are relatively easier to construct. It has become an important tool for researchers to study gene function and potential gene therapy applications. In this article, the researchers reported that TALENs can reliably induce somatic mutations in Xenopus embryos, and these mutations can be inherited through germline transmission. The researchers improved the "Golden Gate" strategy of TALEN assembly, generated a product more suitable for RNA transcription, and injected it into Xenopus embryos by microinjection. The researchers constructed eight pairs of TALENs to target 8 Xenopus genes, and confirmed that all resulted in deletion mutations at the targeted site, with an efficiency as high as 95.7%. In addition, the researchers also confirmed that the mutations induced by TALENs can be transmitted to the F1 generation Xenopus through the germ line very efficiently. Combined with simple and reliable PCR technology to detect TALEN-induced mutations, new research results show that TALENs are an effective tool for targeted gene editing or knockout in Xenopus laevis. Recently, the research team of Chen Yonglong and Zhao Hui have carried out extensive cooperation and achieved a series of breakthrough research progress. Not long ago, they obtained the first gene specific for abdominal pancreatic precursor cells. With the help of gene chip screening, the first marker gene specifically expressed by abdominal pancreatic precursor cells, vpp1, the transcription factor hhex was found in the embryos of African clawed frogs to determine the formation of abdominal pancreatic precursor cells. This research result was also published in the recent PNAS magazine. D Section Pales Palisade Fence
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