elisa principle and classification

elisa principle

The basis of ELISA is the immobilization of antigen or antigen and enzyme labeling of antigen or. The antigen bound on the surface of the solid phase carrier may still maintain its immunological activity. The enzyme-labeled antigen or antibody retains both its immunological activity and enzyme activity. During the measurement, the test specimen (the antibody or antigen in it) is reacted with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid phase carrier is separated from other substances in the liquid by washing. Enzyme-labeled antigens or antibodies are added, and are also bound to the solid-phase carrier through the reaction. At this time, the amount of enzyme on the solid phase is proportional to the amount of test substance in the specimen. After adding the substrate for the enzyme reaction, the substrate is catalyzed by the enzyme into a colored product. The amount of the product is directly related to the amount of the test substance in the specimen, so qualitative or quantitative analysis can be performed according to the depth of the color. Due to the high catalytic efficiency of the enzyme, the results of the immune response are indirectly amplified, making the assay method highly sensitive.

2. Types of ELISA

ELISA can be used to measure antigens and antibodies. There are three necessary in this measurement method: (1) solid phase probiotic or antibody, namely "immunosorbent"; (2) enzyme-labeled antigen or antibody, called "conjugate" ); (3) Substrate for enzyme reaction. According to the source and the situation of the specimen and the specific conditions of the test, various types of test methods can be designed

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D-Arginine

D-arginine has an important role in the medical and pharmaceutical industries, as it is used to treat and relieve hypertension, inhibit the spread of cancer, and relieve the excessive and rapid secretion of growth hormone. In addition, D-arginine is also widely used in basic biochemical research, for example, it can be used as a component of peptides, proteins and drugs as well as involved in some biocatalytic reactions.

D-arginine,D-Arg-OH,157-06-2,C6H14N4O2

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