purpose of usage: Experimental principle: Specimen requirements: Precautions: Storage conditions and validity period:
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The rat thyrotropin ELISA kit is used to determine the content of thyrotropin (TSH) in rat serum, plasma and related liquid samples. Order Hotline, 13296070348
The rat thyrotropin ELISA kit uses the double antibody sandwich method to determine the level of rat thyrotropin (TSH) in the specimen. Microporous plates were coated with purified rat thyrotropin (TSH) antibody to make solid-phase antibodies. Thyrotropin (TSH) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled thyrotropin ( TSH) antibody binds to form antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the thyrotropin (TSH) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat thyrotropin (TSH) in the sample was calculated by a standard curve.
1. Specimens are extracted as soon as possible after collection, and extraction is performed according to relevant literature, and experiments should be conducted as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ° C, but repeated freezing and thawing should be avoided.
2. The samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
1. The kit should be taken out of the refrigerated environment and equilibrated at room temperature for 15-30 minutes before use. If the enzyme-coated plate is unsealed after opening, the strip should be stored in a sealed bag.
2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing.
3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples.
4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the substance to be tested in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with a certain multiple (n times) of the sample diluent and then determine it. Multiple (× n × 5).
5. The sealing film is limited to one-time use to avoid cross-contamination.
6. Please keep the substrate away from light.
7. Strictly follow the instructions, and the test results must be determined by the microplate reader.
8. All samples, washing liquids and various wastes should be treated as infectious agents.
9. The components of different batches of this reagent shall not be mixed.
10. If there is any difference with the English manual, the English manual shall prevail.
1. Rat thyrotropin ELISA kit storage :; 2-8 ℃.
2. Validity: 6 months