The chlorophyll meter can instantly measure the relative chlorophyll content or "greenness" of the plant, so that you can understand the true nitro requirements of the plant and help you understand the lack of soil nitro or the excessive application of nitrogen. You can use this instrument to increase the utilization of nitrogen fertilizer and protect the environment (preventing the application of excessive nitrogen fertilizer to contaminate the environment, especially the water source). work process Calculation Two LED light sources emit two kinds of light, one is red light (peak wavelength 650nm), one is infrared light (940nm), two kinds of light penetrate the blade, hit the receiver, the light signal is converted into analog signal, analog signal It is amplified by an amplifier and converted to a digital signal by an analog/digital converter. The digital signal is used by the microprocessor to calculate the SPAD value and display it on the display and automatically store it in the memory. The calibration of the SPAD502 chlorophyll meter measurements is done by the following procedure. (1) During the calibration process, the measuring head does not sandwich the sample, the two LEDs emit light sequentially, and the received light is converted into an electrical signal, and the ratio of the light intensity is used for calculation. (2) After the sample is clamped by the measuring head, the two LEDs emit light again, and the light transmitted through the blade hits the receiver and is converted into an electrical signal, and the intensity ratio of the transmitted light is calculated. (3) The values ​​of steps 1 and 2 are used to calculate the SPAD measurement, which is the current chlorophyll relative content of the clamped sample leaves. Experimental Results Calculation: The measured absorbance values ​​were substituted into the following formula: Ca = 13.95 A665 - 6.88 A649; Cb = 24.96 A649 - 7.32 A665. From this, the concentrations of chlorophyll a and chlorophyll b (Ca, Cb: mg/L) can be obtained, and the sum of the two is the concentration of total chlorophyll. Finally, according to the following formula, the content of chlorophyll in plant tissues can be further determined: Chlorophyll content (mg/g) = [concentration of chlorophyll x volume of extract × dilution factor] / fresh weight (or dry weight) of the sample. Preparation before measurement 1. Battery installation: Unscrew the battery cover on the lower part of the SPAD502 and install 2 AA batteries in the positive direction; then screw on the cover. Alkaline batteries and carbon-zinc batteries can be used, and batteries of different nature or different amounts of electricity cannot be used. 2, using the reading verification card (1) Turn on the switch and press the "1 DATA DELETE" button and the "DATA RECALL" button at the same time. The instrument enters the check mode, and the screen immediately displays "CH" and then goes to the "CAL" state. (2) Calibrate until the icon appears, indicating that the calibration is complete. (3) Move the depth slider (4) Insert the reading verification card and press the finger press until a “click†sound is heard and the measured value is displayed on the screen. (5) Repeat the measurement reading check card several times. (6) Press the "AVERAGE" button to find the average value of the measurement. The average value displayed on the screen should be within the range of the reading verification card. If it is not in the range, please clean the transmitting window and receiving window, starting from step 1. Repeat the measurement and if it is still out of range, the machine may need to be repaired. (7) Turn off the power, then turn it on again, and measure normally. Special Note l The reading verification card can only be used in inspection mode. l Only a random reading check card (the same serial number as the machine) provides an accurate value. l The reading verification card cannot be used outdoors and cannot be used in direct sunlight, high temperature and high humidity. l When the measured value is different from the value on the reading check card, it cannot be corrected by inputting the compensation value. l Do not touch the glass surface of the reading verification card. If it is dirty, use a wet, soft cloth. l The reading verification card should be protected in the accessory bag and should not be placed in a high temperature or high humidity environment. maintain (1) SPAD502 is waterproof. It can be used as usual on rainy days. Wipe it off with a soft cloth after use, but the machine should never be soaked in water or washed with water. (2) Do not vibrate the instrument vigorously. Do not force the display and measuring head (3) Do not place the instrument in the sun or near a heat source. (4) Shut down after use. (5) If the instrument is dirty, wipe it off with a dry cloth. Do not touch the surface of the instrument with alcohol or chemicals. (6) Do not disassemble the instrument. (7) The storage temperature should be -20~55°C, and should not be placed under high temperature and high humidity conditions. It should be put together with some desiccant. (8) If the storage period is more than two weeks, the battery should be taken out. working principle The measured value is calculated by calculating the amount of light transmitted by the blade in two different wavelength regions, where chlorophyll is not the same for light absorption. These two areas are the red zone (higher absorption of light and not affected by carotene) and the infrared zone (very low absorption of light). The Spad value refers to the relative content of chlorophyll. The value of the plant nitro can be understood by the value of the spad, and the content of nitrogen fertilizer can also be derived. Because the value of the spad is related to the chlorophyll content of the plant, so long as the chlorophyll content of the plant is measured, the value of the spad can be known. Chlorophyll is the main material that absorbs light. Different wavelengths of light and different absorption of leaves, so the relative amount of current chlorophyll in leaves is determined by the transmission coefficient in two wavelength ranges. The SPAD-502 chlorophyll meter evaluates the relative content of chlorophyll in the current leaves by measuring the absorbance of the leaves in the two wavelength bands. measuring The measuring area of ​​the SPAD502 chlorophyll meter is only 2mm*3mm (thickness does not exceed 1.2mm). The center line indicates the center of the measured area. 1, calibration. Calibration is required every time you turn it on. Please follow the procedure below. (1) Turn on the power (2) Without placing the sample, press the probe until you hear a click, and the screen displays N=0 -- indicating that the calibration is complete. (3) If the beeping continues, the icon appears, indicating that the calibration was not completed correctly, and press (2) to repeat the calibration. If the icon appears, the launch window or receiving window needs to be cleaned. After cleaning, press (2) to recalibrate. 2. Place the blade into the measuring head (1) Make sure the sample completely covers the receiving window. (2) Do not measure too thick samples, such as veins. If you have samples with more veins, measure them and average them. (3) If the emission window or receiving window is dirty, the measurement is not accurate and must be cleaned first. (4) Avoid direct sunlight to the instrument to avoid affecting the measurement. (5) Close the measuring head and press the finger pressing table until you hear a “click†sound. The measurement results will be displayed on the screen and stored automatically. (6) If you hear a continuous beep, the measuring head is not tightly closed, or the sample is too thick or too thin, repeat the measurement in steps 2 and 3 until the end of the measurement. (7) If the displayed result is flashing (or no decimal point), the measurement result is greater than 50 (100), and the accuracy of the result is not guaranteed. 3, data storage (1) SPAD502 is automatically stored after the measurement, the storage space is 30 data, when the storage is full, the first data will be deleted, the new data is stored in the 30th position, that is, the first one is deleted, then the original 2~30 becomes 1~29. When the machine is turned off, all data is deleted. (2) Average: All stored data is averaged. (3) Empty: All data is deleted. (4) Delete current data: delete the currently displayed data (5) Browse data: Display previous data. 4, enter the compensation value The compensation value is calculated by the user according to his own needs, and the revision is based on the standardization of several instruments. The compensation value can be set from -9.9 to 9.9. After the compensation value is input, the data is calculated according to the following formula: Display value = measured value + compensation value (1) Enter the compensation value mode: Turn on the power and press "AVERAGE" and "DATA RECALL" simultaneously. The LCD displays the previously entered compensation value. (2) Set the compensation value: set the compensation value by pressing the "ALL DATA CLEAR" button and the "1 DATA DELETE" button. Each time the "ALL DATA CLEAR" button is pressed, the compensation value is increased by 0.1, and each time the "1 DATA DELETE" button is pressed. The compensation value is reduced by 0.1. The compensation value range can be set from -9.9 to 9.9. The reading check card cannot be used to determine the compensation value. (3) Compensation value saving: Press the "AVERAGE" button, the displayed compensation value will be saved and the input will be completed. Shut down, then turn it on again, the compensation value is activated and you can start measuring. 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