1, the setting of the sterile room The sterile room should be set up according to the principles of economy and science. The basic requirements are as follows: (1) The sterile room should have two inner and outer rooms, the inner room is a sterile room, and the outer space is a buffer room. The volume of the room should not be too large for air sterilization. The inner area is 2×2.5=5m2, the outer area is 1×2=2m2, and the height is 2.5m or less. All should have a ceiling. (2) There should be a sliding door in the interior to reduce the fluctuation of the air. The door should be placed at the farthest position from the workbench; the outer door should also be used as a sliding door, which should be located at the farthest distance from the inside. . (3) On the wall or "sparing fan" between the inner and outer compartments, a small window shall be opened for the passage of the internal and external articles necessary for inoculation to reduce the number of times people enter and exit and reduce the degree of pollution. The small window is 60cm wide, 40cm high and 30cm thick, and the window sash is hung inside and outside. (4) The volume of the sterile room is small and rigorous. After using for a period of time, the indoor temperature is very high, so a ventilation window should be provided. The ventilating window shall be provided on the ceiling at the entrance of the inner chamber (ie, the position farthest from the table), preferably a double-layer structure, the outer layer is a louver, and the inner layer may be a sash. The ventilating window can be opened after use in the inner chamber and before sterilization to circulate air. It is conditional to install a constant temperature and humidity machine. 2. Sterile indoor equipment and utensils (1) The workbench in the sterile room requires smooth surface and table level regardless of material or purpose. (2) Install an ultraviolet lamp (30W in total) in the inner and outer chambers. The UV lamp inside the room should be installed directly above the working seat, 2m from the ground, and the UV lamp in the outside room can be installed in the center of the outer room. (3) The outer room should have special overalls, shoes, caps, masks, porcelain basins and towels with Suer water, hand-held sprayers and 5% carbolic acid solution. (4) The inner chamber should have alcohol lamps, commonly used inoculation tools, stainless steel knives, scissors, tweezers, 70% alcohol cotton balls, industrial alcohol, glass slides, special crayons, notebooks, pencils, label paper, glue, Waste baskets, etc. 3. Sterilization and disinfection of sterile room (1) Fumigation: This is a measure of thorough sterilization of the sterile room. The sterile room should be used for a long time. If the pollution is serious, it should be fumigated. It can be fumigated with formaldehyde, lactic acid or sulfur. (2) Spray: Before each use of the sterile room. Spray can promote the sedimentation of particles and microorganisms in the air, prevent the dust on the table and the ground, and have a bactericidal effect. Spray with 5% carbolic acid. (3) Ultraviolet irradiation: before each use of the sterile room. Ultraviolet light has a good bactericidal effect. Generally, the UV lamp should be turned on for 30 to 60 minutes. 4, sterile room work procedures (1) Sterilization in a sterile room. The ultraviolet lamp was turned on for more than 30 minutes before each use, or 5% carbolic acid was sprayed on the inner and outer chambers 30 minutes before use. (2) After washing hands with soap, carry the required equipment into the outer room; replace the sterilized overalls, work caps and work shoes in the outer room, wear a mask, and then dip the hands with 2% phenolic soap for 2 minutes. . (3) Move all kinds of required items into the inner room and check them in place. Spray with 5% carbolic acid above the work surface and the operator's station space, return to the outer room, and then enter the inner room after 5-10 minutes. (4) Before the inoculation operation, wipe the hand with 70% alcohol cotton ball; when performing aseptic operation, the action should be gentle, try to reduce air fluctuations and ground dust. (5) Safety should be paid attention to during work. If the cotton plug is on fire, hold it with your hand or wrap it with a damp cloth. Do not blow it with your mouth to avoid burning up. If the bacteria culture spills or breaks the bacteria container, apply it with a rag impregnated with 5% carbolic acid. Wipe the countertop or the floor with a rag soaked with 5% carbolic acid, and wipe the hand with an alcohol cotton ball before continuing. (6) At the end of the work, immediately clean the countertops. After all the items and wastes that should not be stored in the sterile room are taken out of the sterile room, spray the sterile room with 5% carbolic acid or open the UV lamp for 30 minutes. 5, constant temperature culture room (1) Setting of the culture room (a) The culture room should have two inner and outer chambers, the inner chamber is the culture chamber, and the outer chamber is the buffer chamber. The volume of the room should not be large, in order to facilitate air sterilization. The inner chamber area is about 3.2×4.4=14m2, the outer chamber area is about 3.2×1.8=6m2, and the height is about 2.5m. It should have ceiling. (b) Vents with air filtration means shall be provided in the upper part of the wall separating the inner and outer chambers. (c) In order to meet the microbiological temperature requirements, a constant temperature and humidity machine is required. (d) Both the inner and outer chambers should be fitted with UV lamps in the center of the chamber for sterilization. (2) Cultivating indoor equipment and appliances (a) The inner chamber is usually equipped with a culture rack and a shaker (shaker). Commonly used shaker machines are available in rotary or reciprocating type. (b) The outer room shall have special overalls, shoes, caps, masks, hand-held sprayers and 5% carbolic acid solution, 70% alcohol cotton balls, etc. (3) Sterilization and disinfection of the culture chamber Sterilization and disinfection measures with the sterile room. Small-scale cultures can be performed in a constant temperature incubator without the use of a constant temperature culture chamber. 6, the general laboratory A place where microbial observation, counting, and physiological and biochemical measurements are performed. The furnishings in the room vary greatly depending on the focus of the work. It is usually equipped with a test bench, a microscope, a cabinet and a stool. The test bench is required to be smooth and smooth, and the test cabinet should be sufficient to accommodate the daily use of utensils and medicines. 7, other requirements of the laboratory The capacity, layout and performance of water, electricity and gas should meet the needs of laboratory work. R-BOX Double walled drawer box system Metal Box Kitchen,Wall Metal Box Kitchen,Soft Close Drawer Box System,Soft Close Drawer Box Foshan city boucheron precision hardware co.,ltd , https://www.chinadrawerbox.com