Solution to liquid chromatography "peak delay" failure

Fault phenomenon:

Peak delay

possible reason:

(1) Low column temperature

(2) Sample solvent selection is not appropriate

(3) Sample overload

(4) Column screen blockage or packing collapse

(5) The other peaks wrapped in the target peak did not reach separation.

Method of exclusion:

(1) Properly raise the column temperature

(2) Use a suitable solvent and optionally use a mobile phase to dissolve.

(3) Reduce the injection volume or reduce the sample concentration

(4) Flush or replace the column (if not specified, the backflush column is generally not recommended)

(5) Change the chromatographic conditions, such as prolonging the peak time

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