method one 1. Dilute. We want to leave the plasma (heparin sodium anticoagulated blood collection tube, 5 ~ 6ml), so separate, remove the plasma and add a volume of physiological saline with red blood cells, usually about 3ml, mix. Shanghai Chuangsai Technology Co., Ltd. provides Heparin sodium salt, product number: B11000262-1g, and the price is 69.3 yuan. 2, separation liquid, do not care how much volume, mainly height, if the centrifuge tube is very thick, it is necessary to add more, a little more centrifuge tube can be added less, the general height should exceed 3cm. 3, separation of blood sources, adult peripheral blood is better, umbilical cord blood and placental blood is not good. Of course, hemolysis and coagulation are even more difficult to distinguish. 4, blood preservation, do not put in the 4 degree refrigerator after blood collection (of course, can not be frozen), normal temperature, avoid light. Do not take more than 12 hours from blood collection to separation, otherwise the effect is not good. 5. Centrifugal speed and time. Different centrifuges, the speed is not the same, the laboratory generally uses a horizontal centrifuge 2500 rpm, 20 minutes, basically no red blood cells, and a lot of lymphocytes. 6, the lymphocyte separation solution does not have to be stored at 4 degrees, normal temperature, whether or not opened. In the past experience, after the separation liquid was placed at 4 degrees, no matter how effective the rewarming effect was, the separation effect was not as good as before. Shanghai Chuangsai Technology provides lymphocyte separation solution, BR, commodity number: D16-1013112-250ml, inquiry. Method Two 1. Dilute the anticoagulant sample with 0.85% physiological saline. 2. Take 3-4 ml of lymphocyte separation solution in a clean tube. 3. Slowly add 2 times the diluted peripheral blood volume to the upper layer of the lymphocyte separation solution. 4. Centrifuge at 2000-2500 RPM for 15 minutes. Shanghai Chuangsai Technology Co., Ltd. provides E23-TD4M blood cell washing centrifuge, product number: E23-TD4M-å°, inquiry. 5. Pipette the middle white turbid liquid into the eppendorf tube at -20 ° C for use. Precautions 1, the quality of blood collection is very important, the separation interface is always clear, the blood sample quality is good), with Hanks to double dilution! The separation solution is generally diluted 1/2 of the blood volume! 2, the centrifugation time is very different from the centrifuges in each laboratory! 3. Broken red blood cells with double distilled water for less than one minute. 4, the cleanliness of the test tube is also related. The quality of CANSPEC provides excellent performance, and its excellent quality meets the various needs of different experiments. Standard products, APIs, fetal bovine serum, scientific instruments, laboratory consumables, Shanghai Chuangsai, mass products special promotions, welcome to inquire. ! Replenishing Protein Perm Cream,Curly Hair Perm Cream,Amino Acid Perm Cream,Repair Styling Gel Guangzhou Berfly Cosmetic Co., Ltd , https://www.berflycosmetic.com